Journal: International Journal of Molecular Sciences

Article Title: Biphasic Adaptations of Gastric Epithelial Cells in Chronic H. pylori Infection from Stress to Tolerance

doi: 10.3390/ijms26189016

Figure Lengend Snippet: The migration, invasion capacity, and EMT features of GES-1 cells under H. pylori infection. ( A ) The proliferation capacity of GES-1 WT, GES-1 Hp F1, GES-1 Hp F10, GES-1 Hp F20, and GES-1 Hp F30 cells analyzed by colony formation assay. ( B ) The proliferation capacity of GES-1 WT, GES-1 Hp F1, GES-1 Hp F10, GES-1 Hp F20, and GES-1 Hp F30 cells analyzed by CCK-8 cell proliferation assay. ( C ) The expression of EMT markers (ZO-1, E-cadherin, Snail and N-cadherin) detected by Western blotting. All experiments were performed with 3 biological replicates. One-way ANOVA was used to determine statistical significance in ( A , B ).

Article Snippet: After washing, membranes were incubated with the appropriate secondary antibodies at room temperature for 1 h. The following antibodies were purchased: rabbit anti-E-cadherin antibody (Proteintech, Rosemont, IL, USA, 20874-1-AP, 1:5000 dilution), rabbit anti-N-cadherin antibody (Proteintech, Rosemont, IL, USA, 22018-1-AP, 1:2000 dilution), rabbit anti-ZO-1 antibody (Abcam, Cambridge, UK, ab216880, 1:1000 dilution), rabbit anti-Snail antibody (CST, Danvers, MA, USA, 3879T, 1:1000 dilution), rabbit anti-β-actin antibody (HUABIO, R1102-1, 1:1000 dilution), Nod1 (CST, Danvers, MA, USA, USA, 3545S, diluted 1:1000), Mouse monoclonal to p62 (Abcam, UK, ab56416, 1:1000 dilution), anti-LC3B-II antibody (CST, USA 2775, 1:1000 dilution), and HRP-linked goat anti-rabbit IgG antibody (Solarbio, SE134, 1:5000 dilution).

Techniques: Migration, Infection, Colony Assay, CCK-8 Assay, Proliferation Assay, Expressing, Western Blot